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Regional Mass Spectrometry Centre

The mass spectrometry facility at University de Montréal features state-of-the-art instrumentation for addressing analytical needs in organic, inorganic, environmental, and biological chemistry.

PRESENTATION

Our specialists collaborate to provide both technical guidance and services including:

  • High throughput accurate mass measurement
  • Structure confirmation by MS/MS
  • Mass determination on macromolecules
  • Quantification and separation of mixtures by LCMS

Analyses offered by the mass spectrometry laboratory

  • Determination of nominal mass - available ionisation sources: ESI, APCI, APPI
  • Accurate mass analysis (m/z 50-8000) - available ionisation sources: ESI, APCI, APPI
  • Analysis of high molecular weight molecules (proteins, polymers) - available ionisation sources: ESI
  • Fragmentation pattern generation and analysis on MS/MS spectra (peptides and small molecules) - available ionisation sources: ESI
  • Preparatory LC on reversed phase with fraction collection (collection triggered by mass, UV signal or retention time)
  • Analysis of complex mixtures by tandem separation: LC-MS, LC-MS/MS
  • Chiral and achiral separations by SFC-MS and/or SFC-UV
  • Chiral preparatory SFC with fraction collection triggered by UV

Submission procedure

Submission of samples for accurate mass analysis

Samples should be submitted as approximately 0.1 mg/mL solutions. The most suitable solvents are water, methanol, acetonitrile and dichloromethane. Total sample volume could vary between 0.1 and 1.5 ml. If you need to use other solvents (DMSO for example) or if you dispose of sample volumes smaller than the previously mentioned limit, ask for assistance.

Appropriate vials are available in Room B-4077 of the MIL campus.

Please fill-up the online form for each submitted sample.

 

Submission of samples for SFC

For analytical SFC analyses, the samples should be submitted as a solution around 1 mg/mL. The accepted solvants are methanol, isopropanol and hexanes. Samples containing water, DMF, DMSO or DCM are not suitable for analysis.

For preparative SFC, please submit samples neat.

Please fill-up the online form for each submitted sample.

RATES

The Regional Mass Spectrometry Centre offers a large selection including pricing analysis, each sample and/or time is given in the table below depending on the type of analysis.

AnalysisAcademicPrivate
Masse nominale
Injection directe
$30 / sample$85 / sample
Masse exacte
Injection directe
$60 / sample$160 / sample
LC-MS$60 / injection$160 / injection
LC prep.$100 / heure$275 / injection
SFC$30 / injection
$50 / hour (dévelop. méth.)
$160 / injection
SFC prep.$100 / heure$275 / injection
LC-MS masse exacteRates customized for each project.
LC-MS/MSRates customized for each project.
INSTRUMENTS
LC-TOF - Agilent Technologies
LC-TOF - Agilent Technologies
  • Ionisation sources: ESI, APCI
  • Applications
    - Accurate mass analyses
    - LC-MS purity assessment
    - Degradation products and metabolite identification
    - Intact protein and peptide analyses
Synapt G2-S - Waters
Synapt G2-S - Waters
  • Ionisation sources : ESI, APCI, ASAP
  • Applications
    - Accurate mass analyses
    - Degradation products and metabolite identification/caracterisation by MS/MS fragmentation
    - Intact protein and peptide analyses
    - Ion mobility analysis
Xevo TQ (Triple Quadrupole) - Waters
Xevo TQ (Triple Quadrupole) - Waters
  • Ionisation sources: ESI, APCI
  • Applications
    - Endogenous biomarkers study
    - LC-MS/MS quantitative analyses
         - Pharmacokinetic (DMPK) and drug bioavailability
TSQ Quantum Ultra (Triple Quadrupole) - Thermo Fisher Scientific
TSQ Quantum Ultra (Triple Quadrupole) - Thermo Fisher Scientific
  • Ionisation sources: ESI, APCI
  • Applications
    - Endogenous biomarkers study
    - LC-MS/MS quantitative analyses
         - Pharmacokinetic (DMPK) and drug bioavailability
LC-MSD (Single Quadrupole) - Agilent Technologies
LC-MSD (Single Quadrupole) - Agilent Technologies
  • Ionisation sources: ESI, APCI
  • Applications
    - Nominal mass
    - Purity assessment
    - Following reactions
    - Quantitative analyses
prep-LC-MS (Single Quadrupole) - Waters
prep-LC-MS (Single Quadrupole) - Waters
  • Ionisation source: ESI
  • Range: 50-1000mg
  • Applications
    - Autopurification
         - Collection by UV and/or MS
GC-MS (Single Quadrupole) - Agilent Technologies
GC-MS (Single Quadrupole) - Agilent Technologies
  • Ionisation sources : EI, CI
  • Applications
    - Solvent purity assessment
    - Trace solvents identification in drugs
SFC-MS (Single Quadrupole) - Agilent Technologies
SFC-MS (Single Quadrupole) - Agilent Technologies
  • Ionisation sources : ESI, APCI, ES+APCI
  • Columns : OD-H, AD-H, OJ-H, AS-H, Whelk-O, Lux Cellulose (1 à 4), Lux Amylose 2
  • Applications
    - Chiral and achiral analyses
SFC-UV - Agilent Technologies
SFC-UV - Agilent Technologies
  • UV-DAD detection
  • Columns : OD-H, AD-H, OJ-H, AS-H, Whelk-O, Lux Cellulose (1 à 4), Lux Amylose 2
  • Application
    - Chiral analyses
prep-SFC-UV - Thar (Waters)
prep-SFC-UV - Thar (Waters)
  • Applications
    - Purification of chiral compounds (in "g" range)
  • Columns : OD-H, AD-H, OJ-H, AS-H, Lux Cellulose (1 to 4), Lux Amylose 2
USEFUL LINKS
  • Pierre Chaurand
    Associate Professor, Imaging Mass Spectrometry Laboratory

  • Pierre Thibault
    Full Professor, Chair of Proteomics and Bioanalytical Spectrometry
  • NCBI
    National Center for Biotechnology Information
  • ProteinProspector
    Proteomics tools for mining sequence databases in conjunction with mass spectrometry experiments
CONTACT INFORMATION

Alexandra Furtos-Matei, PhD
Mass Spectrometry Facility Manager

Département de chimie
1375 Avenue Thérèse-Lavoie-Roux
Montréal, QC, H2V 0B3

514 343-5614

 

Research assistants

Marie-Christine Tang - 514-34-6111 poste 3944
Louiza Mahrouche - 514-34-6111 poste 0940
Karine Gilbert -514-34-6111 poste 3944

Mass spectrometry lab

514 343-6111, poste 3958 (labo)

 

For all deliveries, please use the following address

Laboratoire de spectrométrie de masse

1375 Avenue Thérèse-Lavoie-Roux, quai de réception
Montréal, QC, H2V 0B3