Regional Mass Spectrometry Centre
The mass spectrometry facility at University de Montréal features state-of-the-art instrumentation for addressing analytical needs in organic, inorganic, environmental, and biological chemistry.
PRESENTATION
Our specialists collaborate to provide both technical guidance and services including:
- High throughput accurate mass measurement
- Structure confirmation by MSn
- Mass determination on macromolecules
- Quantification and separation of mixtures by LCMS
Analyses offered by the mass spectrometry laboratory
- Determination of nominal mass - available ionisation sources: ESI, APCI, APPI, MALDI
- Accurate mass analysis (m/z 50-8000) - available ionisation sources: ESI, APCI, APPI
- Analysis of high molecular weight molecules (proteins, polymers) - available ionisation sources: ESI, MALDI
- Fragmentation pattern generation and analysis on MSn spectra (peptides and small molecules) - available ionisation sources: ESI
- Preparatory LC on reversed phase with fraction collection (collection triggered by mass, UV signal or retention time)
- Analysis of complex mixtures by tandem separation: LC-MS, LC-MS/MS
- Chiral and achiral separations by SFC-MS and/or SFC-UV
- Chiral preparatory SFC with fraction collection triggered by UV
Submission procedure
Submission of samples for accurate mass analysis
Samples should be submitted as approximately 0.1 mg/mL solutions. The most suitable solvents are water, methanol, acetonitrile and dichloromethane. Total sample volume could vary between 0.1 and 1.5 ml. If you need to use other solvents (DMSO for example) or if you dispose of sample volumes smaller than the previously mentioned limit, ask for assistance.
Appropriate vials are available in Room D-660.
You should fill-up a submission form for each sample, save a copy then send it by e-mail. The submission form is available for downloading.
Submission of samples for SFC
For analytical SFC analyses, the samples should be submitted as a solution around 1 mg/mL. The accepted solvants are methanol, isopropanol and hexanes. Samples containing water, DMF, DMSO or DCM are not suitable for analysis.
For preparative SFC, please submit samples neat.
The submission for is available for downloading and should be thoroughly filled. It can be submitted with the sample or sent by e-mail.
RATES
The Regional Mass Spectrometry Centre offers a large selection including pricing analysis, each sample and/or time is given in the table below depending on the type of analysis.
| Analysis | Academic | Private |
|---|---|---|
| Masse nominale Injection directe | $25 / sample | $75 / sample |
| Masse exacte Injection directe | $50 / sample | $150 / sample |
| LC-MS | $50 / injection | $150 / injection |
| MALDI-TOF | $50 / sample | $150 / sample |
| LC prep. | $50 / injection | $250 / injection |
| SFC | $25 / injection $50 / hour (dévelop. méth.) | $150 / injection |
| SFC prep. | $50 / injection | $250 / injection |
| LC-MS masse exacte | Rates customized for each project. | |
| LC-MS/MS | Rates customized for each project. | |
INSTRUMENTS
USEFUL LINKS
- Pierre Chaurand
Associate Professor, Imaging Mass Spectrometry Laboratory - Pierre Thibault
Full Professor, Chair of Proteomics and Bioanalytical Spectrometry
- ExPASy
Protein knowledgebase
- NCBI
National Center for Biotechnology Information
- ProteinProspector
Proteomics tools for mining sequence databases in conjunction with mass spectrometry experiments
- Scientific Instrument Services
Isotope distribution calculator and mass spec plotter
CONTACT INFORMATION
Alexandra Furtos-Matei, PhD
Mass Spectrometry Facility Manager
Department of Chemistry
2900 boul. Édouard-Montpetit, Room D-657
Montréal QC H3T 1J4
Phone: 514 343-5614
Mass Spectrometry Laboratory
514 343-6111, ext. 3958
Office 514 343-6111, ext. 3944