Regional Mass Spectrometry Centre
The mass spectrometry facility at University de Montréal features state-of-the-art instrumentation for addressing analytical needs in organic, inorganic, environmental, and biological chemistry.
PRESENTATION
Our specialists collaborate to provide both technical guidance and services including:
- High throughput accurate mass measurement
- Structure confirmation by MS/MS
- Mass determination on macromolecules
- Quantification and separation of mixtures by LCMS
Analyses offered by the mass spectrometry laboratory
- Determination of nominal mass - available ionisation sources: ESI, APCI, APPI
- Accurate mass analysis (m/z 50-8000) - available ionisation sources: ESI, APCI, APPI
- Analysis of high molecular weight molecules (proteins, polymers) - available ionisation sources: ESI
- Fragmentation pattern generation and analysis on MS/MS spectra (peptides and small molecules) - available ionisation sources: ESI
- Preparatory LC on reversed phase with fraction collection (collection triggered by mass, UV signal or retention time)
- Analysis of complex mixtures by tandem separation: LC-MS, LC-MS/MS
- Chiral and achiral separations by SFC-MS and/or SFC-UV
- Chiral preparatory SFC with fraction collection triggered by UV
Submission procedure
Submission of samples for accurate mass analysis
Samples should be submitted as approximately 0.1 mg/mL solutions. The most suitable solvents are water, methanol, acetonitrile and dichloromethane. Total sample volume could vary between 0.1 and 1.5 ml. If you need to use other solvents (DMSO for example) or if you dispose of sample volumes smaller than the previously mentioned limit, ask for assistance.
Appropriate vials are available in Room B-4077 of the MIL campus.
Please fill-up the online form for each submitted sample.
Submission of samples for SFC
For analytical SFC analyses, the samples should be submitted as a solution around 1 mg/mL. The accepted solvants are methanol, isopropanol and hexanes. Samples containing water, DMF, DMSO or DCM are not suitable for analysis.
For preparative SFC, please submit samples neat.
Please fill-up the online form for each submitted sample.
RATES
The Regional Mass Spectrometry Centre offers a large selection including pricing analysis, each sample and/or time is given in the table below depending on the type of analysis.
Analysis | Academic | Private |
---|---|---|
Masse nominale Injection directe | $30 / sample | $85 / sample |
Masse exacte Injection directe | $60 / sample | $160 / sample |
LC-MS | $60 / injection | $160 / injection |
LC prep. | $100 / heure | $275 / injection |
SFC | $30 / injection $50 / hour (dévelop. méth.) | $160 / injection |
SFC prep. | $100 / heure | $275 / injection |
LC-MS masse exacte | Rates customized for each project. | |
LC-MS/MS | Rates customized for each project. |
INSTRUMENTS
LC-TOF - Agilent Technologies

- Ionisation sources: ESI, APCI
- Applications
- Accurate mass analyses
- LC-MS purity assessment
- Degradation products and metabolite identification
- Intact protein and peptide analyses
Synapt G2-S - Waters

- Ionisation sources : ESI, APCI, ASAP
- Applications
- Accurate mass analyses
- Degradation products and metabolite identification/caracterisation by MS/MS fragmentation
- Intact protein and peptide analyses
- Ion mobility analysis
Xevo TQ (Triple Quadrupole) - Waters

- Ionisation sources: ESI, APCI
- Applications
- Endogenous biomarkers study
- LC-MS/MS quantitative analyses
- Pharmacokinetic (DMPK) and drug bioavailability
TSQ Quantum Ultra (Triple Quadrupole) - Thermo Fisher Scientific

- Ionisation sources: ESI, APCI
- Applications
- Endogenous biomarkers study
- LC-MS/MS quantitative analyses
- Pharmacokinetic (DMPK) and drug bioavailability
LC-MSD (Single Quadrupole) - Agilent Technologies

- Ionisation sources: ESI, APCI
- Applications
- Nominal mass
- Purity assessment
- Following reactions
- Quantitative analyses
prep-LC-MS (Single Quadrupole) - Waters

- Ionisation source: ESI
- Range: 50-1000mg
- Applications
- Autopurification
- Collection by UV and/or MS
GC-MS (Single Quadrupole) - Agilent Technologies

- Ionisation sources : EI, CI
- Applications
- Solvent purity assessment
- Trace solvents identification in drugs
SFC-MS (Single Quadrupole) - Agilent Technologies

- Ionisation sources : ESI, APCI, ES+APCI
- Columns : OD-H, AD-H, OJ-H, AS-H, Whelk-O, Lux Cellulose (1 à 4), Lux Amylose 2
- Applications
- Chiral and achiral analyses
SFC-UV - Agilent Technologies

- UV-DAD detection
- Columns : OD-H, AD-H, OJ-H, AS-H, Whelk-O, Lux Cellulose (1 à 4), Lux Amylose 2
- Application
- Chiral analyses
prep-SFC-UV - Thar (Waters)

- Applications
- Purification of chiral compounds (in "g" range) - Columns : OD-H, AD-H, OJ-H, AS-H, Lux Cellulose (1 to 4), Lux Amylose 2
USEFUL LINKS
- Pierre Chaurand
Associate Professor, Imaging Mass Spectrometry Laboratory - Pierre Thibault
Full Professor, Chair of Proteomics and Bioanalytical Spectrometry
- ExPASy
Protein knowledgebase
- NCBI
National Center for Biotechnology Information
- ProteinProspector
Proteomics tools for mining sequence databases in conjunction with mass spectrometry experiments
- Scientific Instrument Services
Isotope distribution calculator and mass spec plotter
CONTACT INFORMATION
Alexandra Furtos-Matei, PhD
Mass Spectrometry Facility Manager
Département de chimie
1375 Avenue Thérèse-Lavoie-Roux
Montréal, QC, H2V 0B3
514 343-5614
Research assistants
Marie-Christine Tang - 514-34-6111 poste 3944
Louiza Mahrouche - 514-34-6111 poste 0940
Karine Gilbert -514-34-6111 poste 3944
Mass spectrometry lab
514 343-6111, poste 3958 (labo)
For all deliveries, please use the following address
Laboratoire de spectrométrie de masse
1375 Avenue Thérèse-Lavoie-Roux, quai de réception
Montréal, QC, H2V 0B3